Ninhydrin yog siv ntau txoj hauv kev bioanalytical tshwj xeeb rau txoj kev tsom xam amino acid. Ninhydrin reacts nrog α-amino pawg ntawm thawj amino acids ua 'Ruhemann's ntshav'. Cov chromophore tsim yog tib yam rau tag nrho cov thawj amino acids.
Vim li cas ninhydrin stain amino acids?
Ninhydrin reacts nrog daim tawv nqaijthiab muab cov xim xiav vim muaj cov amino acids hauv daim tawv nqaij. Nws nrhiav tau ammonia thiab amines. Ninhydrins kuj tseem siv los kuaj xyuas cov proteins vim tias muaj cov amine ua ib pawg ua haujlwm.
Ninhydrin stain ua haujlwm li cas?
Ninhydrin yog cov tshuaj reagent siv dav tshaj plaws rau nrhiav pom cov ntiv tes latent rau ntawm qhov ntxeem tau xws li ntawv thiab duab los qhia Lub compound reacts nrog cov amino acid (eccrine) tivthaiv ntawm tus ntiv tes tso nyiaj kom muab ib tug tsaus liab doog khoom hu ua Ruhemann lub ntshav (Daim duab 4).
Ninhydrin stain dab tsi?
Ninhydrin (2, 2-dihydroxyindane-1, 3-dione) yog cov tshuaj siv los kuaj xyuas ammononia lossis thawj thiab theem nrab amines… Ninhydrin feem ntau yog siv los kuaj cov ntiv tes, raws li lub davhlau ya nyob twg amines ntawm lysine residues nyob rau hauv peptides thiab proteins sloughed tawm nyob rau hauv cov ntiv tes react nrog ninhydrin.
Yuav ua li cas yog lub tshuab ntawm ninhydrin kuaj ntawm cov amino acids?
Teb: Lub ninhydrin cov tshuaj tiv thaiv mechanism yog qhov tseem ceeb an oxidation thiab txo cov tshuaj tiv thaiv txheej txheem Thaum peb ntxiv tee ntawm ninhydrin tov rau cov qauv kuaj, ninhydrin ua tus neeg sawv cev oxidizing.. Nws reacts nrog cov amino pawg ntawm cov compound, ua rau delamination.
