Xylene cyanol feem ntau yog siv los ua kev taug qab zas thaum lub sijhawm agarose lossis polyacrylamide gel electrophoresis. Nws muaj qhov tsis zoo me ntsis thiab yuav txav mus rau tib qho kev taw qhia zoo li DNA, tso cai rau tus neeg siv los saib xyuas kev nce qib ntawm cov molecules txav los ntawm gel.
Lub luag haujlwm ntawm xylene cyanol hauv kev thauj khoom yog dab tsi?
Xylene cyanol tuaj yeem siv ua tus cim xim electrophoretic, lossis taug qab dye, los saib xyuas cov txheej txheem ntawm agarose gel electrophoresis thiab polyacrylamide gel electrophoresis. Bromophenol xiav thiab txiv kab ntxwv G kuj tseem siv tau rau lub hom phiaj no.
Qhov txawv ntawm bromophenol xiav thiab xylene cyanol yog dab tsi?
Cov no yog cov tshuaj pleev xim rau koj twv koj xav tau DNA tsiv teb tsaws. Bromophenol xiav migrates yuav luag sib npaug ntawm kev tsiv teb tsaws ntawm ~ 300bp, whereas Xylene Cyanol migrates nyob ib ncig ntawm 3Kb. … Nyob ntawm seb koj xav tau DNA ntev npaum li cas koj tuaj yeem xaiv koj lub ntsej muag xim.
Vim li cas xylene cyanol ntxiv rau DNA kuaj kom khiav gel electrophoresis?
Loading Dye Purpose and Importance
DNA tsis muaj xim, yog li ntxiv cov dyes taug qab rau cov qauv pab koj txiav txim siab tus nqi ntawm qhov sib txawv ntawm cov protein molecules hauv cov gel thaum lub sij hawm electrophoresisPiv txwv ntawm kev thauj khoom dyes uas txav nrog cov qauv DNA suav nrog bromophenol xiav thiab xylene cyanol.
Xim ntawm xylene cyanol yog dab tsi?
Composition: Dej 99.85%, Xylene Cyanol FF 0.10%, Methyl Txiv kab ntxwv, Sodium ntsev 0.05% Boiling Point: Kwv yees li 100 ° C ntom: 1 Melting Point: 0 ° C Xim: Dark xiav- ntsuab kua Lub cev lub cev: Ua kua pH Ntau Yam: 2.9 (liab ntshav) - 4.6 (ntsuab) Cov Lus Qhia Solubility: Miscible Txee Lub Neej:…
