Kev xaiv cov lus qhia rau cov dej tsis muaj nuclease Lawv yog Hom II 18-megohm lim (ntawm lub xeev-of-the-art thim rov qab osmosis pom thiab deionization), ua raws li autoclaving thiab sterile filtration.
Nuclease dawb dej ua dab tsi?
To tiv thaiv DNA thiab RNA cov qauv poob, nws yog qhov tseem ceeb uas cov dej ntshiab huv, tsis muaj nuclease siv rau hauv cov ntawv thov xws li PCR, cDNA synthesis, nucleic acid purification, sequencing, thiab cloning. … Cov hau kev ntawm kev tshem tawm cov nucleases nquag muaj xws li pom thiab kho nrog diethylpyrocarbonate (DEPC).
Puas yog nuclease dawb dej ultrapure?
Nuclease-free dej yog dawb ntawm DNAse thiab RNAse, thiab koom nrog kev kho nrog DEPC (diethylpyrocarbonate) thiab / lossis autoclaving kom inactivate RNAse thiab DNAse. Ultrapure dej, ntawm qhov tod tes, yog tau los ntawm ultrafiltration kom ua tiav qib siab ntawm purity. Nws kuj tsis muaj cov nucleases.
Koj puas tuaj yeem ua dej tsis muaj nuclease?
Txhua Cov Lus Teb (10) Ntxiv 0.1% DEPC rau MilliQ lossis Ob Chav Distilled dej - cia nws zaum hmo ntuj ntawm 37degC thiab tom qab ntawd Autoclave nws. Xyuas kom tseeb tias cov iav uas siv los kuj tau muab yaug nrog dej tib yam lossis kho nrog Chloroform lossis Ci hauv qhov cub cua kub (260degC) rau 4 teev. Nws yuav tsum npaj txhij rau kev siv - ob qho tib si DNase thiab RNase free
Dab tsi yog qhov txawv ntawm nuclease dawb dej thiab RNase dawb dej?
Nuclease free dej txhais tau tias free ntawm ob nuclease. DNase feem ntau tsis ruaj khov. Nws tuaj yeem deactivated los ntawm vortexing lossis cua sov tab sis RNase yog qhov ruaj khov dua. Nyob rau hauv rooj plaub ntawm RT koj yog siv RNase dej dawb lossis nuclease dawb dej.
