Cov txheej txheem:
- Nuclease dawb dej ua dab tsi?
- Puas yog nuclease dawb dej ultrapure?
- Koj puas tuaj yeem ua dej tsis muaj nuclease?
- Dab tsi yog qhov txawv ntawm nuclease dawb dej thiab RNase dawb dej?
Video: Koj puas tuaj yeem haus dej tsis muaj nuclease?
2024 Tus sau: Fiona Howard | [email protected]. Kawg hloov kho: 2024-01-10 06:38
Kev xaiv cov lus qhia rau cov dej tsis muaj nuclease Lawv yog Hom II 18-megohm lim (ntawm lub xeev-of-the-art thim rov qab osmosis pom thiab deionization), ua raws li autoclaving thiab sterile filtration.
Nuclease dawb dej ua dab tsi?
To tiv thaiv DNA thiab RNA cov qauv poob, nws yog qhov tseem ceeb uas cov dej ntshiab huv, tsis muaj nuclease siv rau hauv cov ntawv thov xws li PCR, cDNA synthesis, nucleic acid purification, sequencing, thiab cloning. … Cov hau kev ntawm kev tshem tawm cov nucleases nquag muaj xws li pom thiab kho nrog diethylpyrocarbonate (DEPC).
Puas yog nuclease dawb dej ultrapure?
Nuclease-free dej yog dawb ntawm DNAse thiab RNAse, thiab koom nrog kev kho nrog DEPC (diethylpyrocarbonate) thiab / lossis autoclaving kom inactivate RNAse thiab DNAse. Ultrapure dej, ntawm qhov tod tes, yog tau los ntawm ultrafiltration kom ua tiav qib siab ntawm purity. Nws kuj tsis muaj cov nucleases.
Koj puas tuaj yeem ua dej tsis muaj nuclease?
Txhua Cov Lus Teb (10) Ntxiv 0.1% DEPC rau MilliQ lossis Ob Chav Distilled dej - cia nws zaum hmo ntuj ntawm 37degC thiab tom qab ntawd Autoclave nws. Xyuas kom tseeb tias cov iav uas siv los kuj tau muab yaug nrog dej tib yam lossis kho nrog Chloroform lossis Ci hauv qhov cub cua kub (260degC) rau 4 teev. Nws yuav tsum npaj txhij rau kev siv - ob qho tib si DNase thiab RNase free
Dab tsi yog qhov txawv ntawm nuclease dawb dej thiab RNase dawb dej?
Nuclease free dej txhais tau tias free ntawm ob nuclease. DNase feem ntau tsis ruaj khov. Nws tuaj yeem deactivated los ntawm vortexing lossis cua sov tab sis RNase yog qhov ruaj khov dua. Nyob rau hauv rooj plaub ntawm RT koj yog siv RNase dej dawb lossis nuclease dawb dej.
Pom zoo:
Koj puas tuaj yeem haus dej kwj dej?
Tsis txhob haus dej los ntawm tej yam ntuj tso uas koj tsis tau ntxuav, txawm tias dej yuav huv si. Cov dej hauv cov kwj dej, dej los yog pas dej yuav zoo li huv si, tab sis nws tseem tuaj yeem muaj cov kab mob, kab mob, thiab kab mob uas tuaj yeem ua rau muaj kab mob hauv dej, xws li cryptosporidiosis lossis giardiasis .
Koj puas tuaj yeem haus dej haus zoo?
Yog, ua tus tswv lub qhov dej ntiag tug, koj yog lub luag haujlwm kuaj koj lub qhov dej kom ntseeg tau tias cov dej nyab xeeb rau haus. EPA yog lub luag haujlwm los xyuas kom meej tias cov dej hauv pej xeem hauv Tebchaws Meskas muaj kev nyab xeeb.
Koj puas tuaj yeem haus dej haus cawv?
Dej sau los ntawm lub tshuab cua txias yog condensation rub los ntawm huab cua hauv koj lub tsev. Nws yuav tsum tsis txhob noj, thiab tsis muaj kev nyab xeeb rau haus, vim nws muaj cov khoom tsis huv hauv huab cua hauv koj lub tsev, suav nrog cov tshuaj thiab cov hlau hnyav .
Koj puas tuaj yeem haus dej haus tawm ntawm pewter tankards?
Nco ntsoov tias cov khoom qub qub lossis, qee zaus pheej yig dua sab hnub tuaj pewter, yuav muaj cov hmoov lead. … Sij hawm dhau mus haus dej haus los ntawm lub tank uas ua los ntawm cov hmoov txhuas los yog qib qis ntawm pewter tuaj yeem ua rau koj noj qab haus huv heev, thiab peb pom zoo nrog FDA tias qhov no yog lub tswv yim phem .
Kuv puas tuaj yeem haus dej tsis muaj dej?
Demineralised dej uas tsis tau remineralized, lossis cov dej ntxhia tsawg - hauv qhov pom ntawm qhov tsis muaj lossis tsis muaj cov zaub mov tseem ceeb hauv nws - yog tsis suav tias yog dej haus zoo tshaj, thiab yog li ntawd, nws noj tsis tu ncua yuav tsis muab cov qib txaus ntawm qee cov khoom noj muaj txiaj ntsig .
