Hauv biochemistry, Lineweaver-Burk zajlus (los yog ob chav sib txuas lus) yog ib daim duab sawv cev ntawm Lineweaver-Burk kab zauv ntawm enzyme kinetics, piav qhia los ntawm Hans Lineweaver thiab Dean Burk xyoo 1934.
Dab tsi yog Lineweaver-Burk zaj duab xis qhia?
Cov Lineweaver-Burk zajlus tau siv dav txhawm rau txiav txim siab cov ntsiab lus tseem ceeb hauv enzyme kinetics, xws li Km thiab Vmax, ua ntej muaj ntau lub khoos phis tawj muaj zog thiab cov software tsis yog linear regression.. Lub y-intercept ntawm xws li ib daim duab yog sib npaug rau inverse ntawm Vmax; x-intercept ntawm daim duab sawv cev −1/Km.
kab sib tshuam ntawm Lineweaver-Burk qhov twg?
Txhua Cov Lus Teb (3) Yog tias koj tab tom saib cov tshuaj tiv thaiv monosubstrate, muaj ib rooj plaub uas linear Lineweaver-Burk cov phiaj xwm sib tshuam hauv quadrant 1. Qhov no yog ib qho ntawm 6 kis ntawm nonlinear hyperbolic inhibition, case 6: hyperbolic noncompetitive inhibition.
Vim li cas Lineweaver-Burk yog qhov tseeb dua?
Piv txwv li; Lineweaver-Burke zaj dab neeg, zaj dab neeg nyiam tshaj plaws los ntawm cov kws tshawb fawb, muaj ob qhov zoo sib xws ntawm Michaelis-Menten zaj dab neeg, nyob rau hauv uas nws muab qhov tseeb kwv yees ntau dua ntawm V max thiab cov ntaub ntawv raug ntau ntxiv txog inhibition.. Nws nce qhov tseeb los ntawm linearizing cov ntaub ntawv
Vim li cas Lineweaver-Burk yog qhov tseem ceeb?
Siv Lineweaver–Burk Plot
Siv los txiav txim cov ntsiab lus tseem ceeb hauv enzyme kinetics , xws li Kmand Vmax, ua ntej muaj dav dav ntawm cov khoos phis tawj muaj zog thiab cov software tsis-linear regression. Ua kom nrawm, pom kev pom ntawm ntau hom enzyme inhibition.