Cov lus qhia rau thawing hlwb
- Taw cov hlwb khov sai sai (< 1 feeb) hauv da dej 37 ° C.
- Dilute cov hlwb thawed maj mam ua ntej koj incubate lawv, siv pre-warmed loj hlob nruab nrab.
- Plate thawed hlwb ntawm qhov ntom ntom kom ua kom zoo dua qub.
- Ib txwm siv cov txheej txheem aseptic kom zoo thiab ua haujlwm hauv laminar ntws hood.
Vim li cas thawing ntawm hlwb ua tiav?
Nws yog ib qho tseem ceeb rau thaw hlwb kom raug thiaj li muaj peev xwm ntawm kab lis kev caithiab ua kom kab lis kev cai rov qab sai dua. Qee cov tshuaj tiv thaiv cryoprotectants, xws li DMSO, yog tshuaj lom tshaj 4 ° C. Yog li ntawd, nws yog ib qho tseem ceeb uas kab lis kev cai yog thawed sai thiab diluted nyob rau hauv kab lis kev cai nruab nrab kom txo tau cov tshuaj lom.
txheej txheem ntawm thawing yog dab tsi?
Thawing yog txheej txheem ntawm kev noj cov khoom khov los ntawm khov rau qhov kub (feem ntau siab dua 0 ° C) qhov uas tsis muaj dej khov seem, piv txwv li "defrosting". Thawing feem ntau suav hais tias yog qhov kev thim rov qab ntawm cov txheej txheem khov.
Yuav ua li cas yog txoj kev zoo tshaj plaws kom khov hlwb?
Txias lub hlwb maj mam los ntawm kev txo qhov kub ntawm kwv yees li 1 ° C ib feeb twg siv tswj tus nqi cryo-freezer lossis lub thawv txias txias xws li Mr. Frosty,”muaj los ntawm Thermo Scientific Nalgene labware (Nalge Nunc). Ib txwm siv qhov nruab nrab khov uas pom zoo.
Yuav ua li cas thaw ib lub cell thawj?
So sab nraud ntawm lub vial ntawm cov hlwb nrog 70% ethanol lossis isopropanol. Nyob rau hauv lub txee biosafety, twist lub hau ib lub quarter-tig kom txo qis sab hauv thiab rov qab. Ceev ceev thaw hlwb hauv 37 ° C dej da dej los ntawm maj mam swirling lub vial. Tshem lub vial thaum cov dej khov me me.
