Cov txheej txheem:
- Cov lus qhia rau thawing hlwb
- Vim li cas thawing ntawm hlwb ua tiav?
- txheej txheem ntawm thawing yog dab tsi?
- Yuav ua li cas yog txoj kev zoo tshaj plaws kom khov hlwb?
- Yuav ua li cas thaw ib lub cell thawj?
Video: Thaum thawing tsiaj hlwb koj yuav tsum?
2024 Tus sau: Fiona Howard | [email protected]. Kawg hloov kho: 2024-01-10 06:38
Cov lus qhia rau thawing hlwb
- Taw cov hlwb khov sai sai (< 1 feeb) hauv da dej 37 ° C.
- Dilute cov hlwb thawed maj mam ua ntej koj incubate lawv, siv pre-warmed loj hlob nruab nrab.
- Plate thawed hlwb ntawm qhov ntom ntom kom ua kom zoo dua qub.
- Ib txwm siv cov txheej txheem aseptic kom zoo thiab ua haujlwm hauv laminar ntws hood.
Vim li cas thawing ntawm hlwb ua tiav?
Nws yog ib qho tseem ceeb rau thaw hlwb kom raug thiaj li muaj peev xwm ntawm kab lis kev caithiab ua kom kab lis kev cai rov qab sai dua. Qee cov tshuaj tiv thaiv cryoprotectants, xws li DMSO, yog tshuaj lom tshaj 4 ° C. Yog li ntawd, nws yog ib qho tseem ceeb uas kab lis kev cai yog thawed sai thiab diluted nyob rau hauv kab lis kev cai nruab nrab kom txo tau cov tshuaj lom.
txheej txheem ntawm thawing yog dab tsi?
Thawing yog txheej txheem ntawm kev noj cov khoom khov los ntawm khov rau qhov kub (feem ntau siab dua 0 ° C) qhov uas tsis muaj dej khov seem, piv txwv li "defrosting". Thawing feem ntau suav hais tias yog qhov kev thim rov qab ntawm cov txheej txheem khov.
Yuav ua li cas yog txoj kev zoo tshaj plaws kom khov hlwb?
Txias lub hlwb maj mam los ntawm kev txo qhov kub ntawm kwv yees li 1 ° C ib feeb twg siv tswj tus nqi cryo-freezer lossis lub thawv txias txias xws li Mr. Frosty,”muaj los ntawm Thermo Scientific Nalgene labware (Nalge Nunc). Ib txwm siv qhov nruab nrab khov uas pom zoo.
Yuav ua li cas thaw ib lub cell thawj?
So sab nraud ntawm lub vial ntawm cov hlwb nrog 70% ethanol lossis isopropanol. Nyob rau hauv lub txee biosafety, twist lub hau ib lub quarter-tig kom txo qis sab hauv thiab rov qab. Ceev ceev thaw hlwb hauv 37 ° C dej da dej los ntawm maj mam swirling lub vial. Tshem lub vial thaum cov dej khov me me.
Pom zoo:
Koj puas yuav rov khov qaib tom qab thawing?
Tebchaws Asmeskas Lub Tsev Haujlwm Saib Xyuas Kev Ua Liaj Ua Teb (USDA) qhia: Thaum zaub mov yog thawed hauv lub tub yees, nws muaj kev nyab xeeb kom rov khov dua yam tsis tau ua noj, txawm tias tej zaum yuav poob. zoo vim cov ya raws poob los ntawm thawing.
Koj puas yuav tsum raug xa mus rau kws kho mob hlwb?
Tus kws kho mob hlwb tau txais daim ntawv kho mob thiab tuaj yeem sau tshuaj, nrog rau kev kho mob. … Feem ntau koj tsis tas yuav xa mus rau tus kws kho mob hlwb, tab sis mus ntsib kws kho mob hlwb, koj yuav xav tau kev xa mus los ntawm koj tus kws kho mob .
Yuav tsum kho koj txoj haujlwm yuav tsum tau ua los ntawm tus thawj saib xyuas?
Teb: Kev ntxhov siab ntawm kev ua haujlwm, kev kho koj txoj haujlwm yuav tsum xav tau los ntawm tus thawj saib xyuas thiab nws yuav tsum tau ua raws li qhov ua yuam kev. Kev piav qhia: Yog lawm, tus thawj saib xyuas yuav tsum tau saib xyuas qhov ua yuam kev vim yog ib txoj haujlwm tseem ceeb ntawm tus thawj saib xyuas .
Kuv yuav tsum muag cov tsiaj txhu hauv kev hla tsiaj?
Ib txoj hauv kev zoo tshaj plaws los ua Tswb thaum ntxov ntawm Tsiaj Hla New Horizons yog muag ntses thiab kab Pom tseeb, koj yuav xav pub hom tshiab uas koj pom rau Blathers (ntau ntxiv ntawm qhov no), tab sis siv qee lub sijhawm nuv ntses thiab ntes kab yuav ua rau koj tau nyiaj sai sai .
Yuav tsum tau hloov pauv hauv 24 teev tom qab thawing?
Thawing yuav tsum tau 15-30 feeb nyob ntawm seb muaj pes tsawg chav nyob thawed FFP/FP yog relabeled li Thawed Plasma. Thaum thawed, muab tso rau hauv Blood Bank lub tub yees thiab hloov tsis pub dhau 5 hnub yog tias khaws cia hauv qhov kaw, lossis hauv 24 teev yog tias lub kaw lus nkag.
